Developed by Takashi Sado, National Institute of Genetics at 2004. R1 ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6. Cre/loxP system Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://www.bioreg.kyushu-u.ac.jp/labo/epigenome/index.html" target="_blank">Lab HP (Japanese)</A> <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/feb_2009_mm" target="_blank">Mouse of the Month Feb 2009</A> Xist1loxGFP mice 国立遺伝学研究所・佐渡　敬先生（2004）。R1 ES細胞を用いて作出。 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>・マウスを使用するに当たり寄託者と競合する研究ではない事を確認する。<br>・成果によるパテント等の申請は寄託者と別途協議する。 SA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish GFP cDNA, poly A, phage P1 loxP site, mouse Xist genomic DNA Xist1loxGFPマウス B6;129(Cg)-Xist<tm2Sado> B6;129(Cg)-Xist<tm2Sado> 大学共同利用機関法人情報・システム研究機構国立遺伝学研究所 佐渡　敬 Xist gene knockout mice (Xist<1loxGFP>). Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655). The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must inform the DEPOSITOR the research project using the<br>BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to<br>avoid the conflict of interest with the DEPOSITOR.<br>The RECIPIENT should contact the DEPOSITOR in the case of application for<br>any patents with the results from these mice. Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] C（3〜6か月） C (3-6 months) Res. Org. Info. System Xist遺伝子のノックアウトマウス(Xist<1loxGFP>)。父親由来のmutant alleleをもつ雌は胚性致死。Tsix遺伝子ノックアウト (Xist<1loxGFP>) (RBRC01949) 、Tsix遺伝子スプライシングノックアウト (Tsix<SA>) (RBRC01949) 、Xist/Tsixダブルノックアウト (X<dc>) (RBRC01950) 、Tsix<pA> (RBRC02653) 、Xist<IVS> (RBRC02654) 、Xist<delta> (RBRC02655) 。 <a href='https://brc.riken.jp/mus/pcr01260'>Genotyping protocol -PCR-</a> Fluorescent Proteins/lacZ System true Takashi SADO RBRC01260